HSP47 Antibody Pair - BSA and Azide free (ab253318)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
Overview
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Product name
HSP47 Antibody Pair - BSA and Azide free
See all Hsp47 kits -
Assay type
ELISA set -
Range
218.75 pg/ml - 14000 pg/ml -
Product overview
The Antibody Pair can be used to quantify HSP47. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270882), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests HSP47 Capture Antibody (unconjugated) 1 x 100µg HSP47 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
Involvement in disease
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Alternative names
- 47 kDa heat shock protein
- 47 kDa heat shock protein precursor
- Arsenic transactivated protein 3
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Images
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Sandwich ELISA - HSP47 Antibody Pair - BSA and Azide free (ab253318)To learn more about the advantages of recombinant antibodies see here.
