HSP47 ELISA Kit (ab270882)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 119.23 pg/ml
- Range: 218.75 pg/ml - 14000 pg/ml
- Sample type: Cell culture extracts, Cell Lysate, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
Overview
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Product name
HSP47 ELISA Kit
See all Hsp47 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 5.9% Inter-assay Sample n Mean SD CV% Extract 3 8.3% -
Sample type
Serum, Cell culture extracts, Cell Lysate, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
119.23 pg/ml -
Range
218.75 pg/ml - 14000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 88% - 90% Cell culture extracts 106 88% - 115% Cell Lysate 107 102% - 111% Cit plasma 82 80% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
HSP47 ELISA kit (ab270882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HSP47 protein in human serum and plasma (citrate) and human, mouse and rat cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate HSP47 with 119.23 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
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Notes
Heat Shock Protein47 (HSP47) is a collagen specific molecular chaperone protein found in the endoplasmic reticulum. HSP47 assists in the triple-helical formation of collagen and is part of the collagen quality control system. Collagen accounts for approximately 30% of the total protein found in vertebrates and is the major component responsible in providing structural support for a number of tissues. HSP47 can be found constitutively expressed in non-stressed cells associated with collagen expression. Increased HSP47 expression has been associated with increase collagen deposition in arteriosclerosis, myocardial infarction, hepatic fibrosis, renal fibrosis, and pulmonary fibrosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests HSP47 Lyophilized Recombinant Protein 2 vials 10X HSP47 Capture Antibody 1 x 600µl 10X HSP47 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
Involvement in disease
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Alternative names
- 47 kDa heat shock protein
- 47 kDa heat shock protein precursor
- Arsenic transactivated protein 3
see all -
Database links
- Entrez Gene: 871 Human
- Entrez Gene: 12406 Mouse
- Entrez Gene: 29345 Rat
- Omim: 600943 Human
- SwissProt: P50454 Human
- SwissProt: P19324 Mouse
- SwissProt: P29457 Rat
- Unigene: 596449 Human
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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HSP47 standard curve comparisonStandard Curve comparison between the original HSP47 SimpleStep ELISA kit (ab202407) and current HSP47 SimpleStep ELISA kit (ab270882).
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Example of HSP47 standard curve in Sample Diluent NS.The HSP47 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native HSP47 in human HepG2, MCF7, and HeLa extract samples based on a 25 µg/mL extract load.The concentrations of HSP47 were measured in duplicate and interpolated from the HSP47 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP47 concentration was determined to be 14.52 ng/mL in HepG2, 14.19 ng/ml in MCF7, and 12.46 ng/mL in HeLa extract samples.
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Interpolated concentrations of native HSP47 in human, mouse, and rat liver homogenate extract samples based on a 125 µg/mL extract load.The concentrations of HSP47 were measured in duplicate and interpolated from the HSP47 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP47 concentration was determined to be 2.16 ng/mL in human, 1.97 ng/ml in mouse, and 8.90 ng/mL in rat liver homogenate extract samples.
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Sandwich ELISA - HSP47 ELISA Kit (ab270882)To learn more about the advantages of recombinant antibodies see here.
