Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17413] to CSDE1/NRU
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-CSDE1/NRU antibody [EPR17413]
See all CSDE1/NRU primary antibodies
Description

Rabbit monoclonal [EPR17413] to CSDE1/NRU
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, HeLa, MCF7, C6, RAW 264.7 and PC-12 cell lysates; Human fetal brain lysate; HeLa cytosolic fraction. IP: HeLa whole cell lysate.
General notes
This product was previously labelled as CSDE1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17413
Isotype

IgG
Research areas

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution + MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution + Human fetal brain lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cells) cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

Lane 1 : HeLa cytosolic fraction
Lane 2 : HeLa membrane (mitochondrial) fraction
Lane 3 : HeLa nuclear fraction

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
The HeLa cell fractions lysate set is ab168542 from Abcam.
Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200663 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/1000 dilution (Panel A) or ab201688 at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200663 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min (Panel A); 10 seconds (Panel B).
Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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