Anti-HIP55 antibody (ab77147)
Key features and details
- Rabbit polyclonal to HIP55
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIP55 antibody
See all HIP55 primary antibodies -
Description
Rabbit polyclonal to HIP55 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human HIP55 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab91635) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: SHSY-5Y; SK N SH; SK N BE; HeLa.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab77147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF WB Application notesICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. -
Sequence similarities
Belongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1. -
Post-translational
modificationsDegraded by caspases during apoptosis. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin. - Information by UniProt
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Database links
- Entrez Gene: 28988 Human
- Entrez Gene: 13169 Mouse
- Entrez Gene: 83527 Rat
- Omim: 610106 Human
- SwissProt: Q9UJU6 Human
- SwissProt: Q62418 Mouse
- SwissProt: Q9JHL4 Rat
- Unigene: 436500 Human
see all -
Alternative names
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
see all
Images
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Western blot - Anti-HIP55 antibody (ab77147)All lanes : Anti-HIP55 antibody (ab77147) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 55 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human Drebrin-like protein (HIP 55). -
Immunocytochemistry/ Immunofluorescence - Anti-HIP55 antibody (ab77147)ICC/IF image of ab77147 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab77147 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HIP55 antibody (ab77147)All lanes : Anti-HIP55 antibody (ab77147) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 55 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human Drebrin-like protein (HIP 55). -
Immunocytochemistry/ Immunofluorescence - Anti-HIP55 antibody (ab77147)ICC/IF image of ab77147 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
