Anti-HIP55 antibody - N-terminal (ab154016)
Key features and details
- Rabbit polyclonal to HIP55 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIP55 antibody - N-terminal
See all HIP55 primary antibodies -
Description
Rabbit polyclonal to HIP55 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within N terminal amino acids 1- 44 of Human HIP55 (Uniprot ID: Q9UJU6).
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Positive control
- HeLa S3 and MOLT4 whole cell lysate; HeLa cells; Human H1299 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP55 antibody - N-terminal (ab154016)Immunohistochemical analysis of paraffin-embedded Human H1299 xenograft tissue labeling HIP55 with ab154016 at 1/100 dilution.
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Western blot - Anti-HIP55 antibody - N-terminal (ab154016)All lanes : Anti-HIP55 antibody - N-terminal (ab154016) at 1/1500 dilution
Lane 1 : HeLa S3 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HIP55 antibody - N-terminal (ab154016)Immunofluorescent analysis of HeLa cells (fixed in iced-cold Methanol for 5 min) labeling HIP55 with ab154016 at 1/500 dilution (green). Right: Blue staining represents cells costained with Hoechst 33343.
