Anti-eIF4G1 antibody (ab264232)
Key features and details
- Rabbit polyclonal to eIF4G1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4G1 antibody
See all eIF4G1 primary antibodies -
Description
Rabbit polyclonal to eIF4G1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit
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Immunogen
Synthetic peptide within Human eIF4G1 aa 250-300. The exact sequence is proprietary.
Database link: Q04637 -
Positive control
- WB: HeLa whole cell lysate; IP: HeLa whole cell lysate; IHC-P: Human ovarian carcinoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4G1 antibody (ab264232)All lanes : Anti-eIF4G1 antibody (ab264232) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Exposure time: 3 seconds4-8% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4G1 antibody (ab264232)Immunohistochemical analysis of FFPE human ovarian carcinoma tissue section labeling eIF4G1 with ab264232 at 1/200 dilution. Detection: DAB.
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Immunoprecipitation - Anti-eIF4G1 antibody (ab264232)eIF4G1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264232 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264232 at 1 µg/ml.
Lane 1: ab264232 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
