Anti-LIM2 antibody [EPR16947] (ab198502)
![Anti-LIM2 antibody [EPR16947] (ab198502)](https://www.abcam.com/ps/products/198/ab198502/Images/ab198502-284784-ab198502WBmousev2.jpg "Anti-LIM2 antibody [EPR16947] (ab198502)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16947] to LIM2
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-LIM2 antibody [EPR16947]
See all LIM2 primary antibodies -
Description
Rabbit monoclonal [EPR16947] to LIM2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse and Rat eyeball lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16947 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LIM2 antibody [EPR16947] (ab198502)All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/50000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-LIM2 antibody [EPR16947] (ab198502)](https://www.abcam.com/ps/products/198/ab198502/Images/ab198502-284783-ab198502WBratv2.jpg)
Western blot - Anti-LIM2 antibody [EPR16947] (ab198502)All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/5000 dilution
Lane 1 : Rat eyeball lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat heart lysate
Lane 4 : Rat kidney lysate
Lane 5 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
-
![Anti-LIM2 antibody [EPR16947] (ab198502)](https://www.abcam.com/ps/products/198/ab198502/Images/ab198502-5-benefits-of-recombinant-antibodies.png)
Anti-LIM2 antibody [EPR16947] (ab198502)