Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

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Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17414] to CSDE1/NRU
Suitable for: ICC/IF, IP, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-CSDE1/NRU antibody [EPR17414]
See all CSDE1/NRU primary antibodies
Description

Rabbit monoclonal [EPR17414] to CSDE1/NRU
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, HEK293, Raw264.7 and HeLa cell lysate. Human testis, mouse and rat lysate. IHC-P: Human breast carcinoma and Human kidney tissue. ICC/IF: HeLa and MCF7 cells. IP: K562 cells.
General notes
This product was previously labelled as CSDE1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17414
Isotype

IgG
Research areas

Western blot - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

All lanes : Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/10000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) lysate
Lane 2 : HEK293 (Human embryonic kidney)
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/1000 dilution + Human testis at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

All lanes : Anti-CSDE1/NRU antibody [EPR17414] (ab201688) at 1/1000 dilution

Lane 1 : Mouse brain
Lane 2 : Rat brain
Lane 3 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on Human breast carcinoma tissue is observed.

Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on Human kidney tissue is observed.

Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing cytoplasmic staining on HeLa cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab201688 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab201688 at 1/1500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1: K562 whole cell extract 10 µg (Input).

Lane 2: ab201688 IP in K562 whole cell extract.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20688 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second
Anti-CSDE1/NRU antibody [EPR17414] (ab201688)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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