Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17414] to CSDE1/NRU - BSA and Azide free
  • Suitable for: IP, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free
    See all CSDE1/NRU primary antibodies

  • Description

    Rabbit monoclonal [EPR17414] to CSDE1/NRU - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    ab236149 is the carrier-free version of ab201688 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236149 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CSDE1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasmic staining on Human breast carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201688).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
    Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

    CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab201688 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab201688 at 1/1500 dilution. 

    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab201688 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20688 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201688).

  • Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
    Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201688).

  • Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
    Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CSDE1/NRU with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). 

    Confocal image showing cytoplasmic staining on HeLa cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201688).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CSDE1/NRU with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201688).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
    Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)

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