Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7900(2)] to TIP-1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free
    See all TIP-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7900(2)] to TIP-1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung and heart tissue lysates. Rat lung and heart tissue lysate. NIH/3T3, A673, LLC1, and C6 whole cell lysates; IHC-P: Human thyroid carcinoma tissue; Flow-cyt: A673 cells.

  • General notes

    ab213403 is the carrier-free version of ab155992 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab213403 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as TAX1BP3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIP-1 with Purified ab155992 at 1/1000 dilution (2.05 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155992).
  • Western blot - Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    Western blot - Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    All lanes : Anti-TIP-1 antibody [EPR7900(2)] (ab155992) at 1/1000 dilution (Purified)

    Lane 1 : A-673 (Human muscle Ewing's Sarcoma) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : LLC1 (Mus musculus lung rounded-looselyattachedorfloating) whole cell lysates
    Lane 4 : Mouse lung lysates
    Lane 5 : Mouse heart lysates
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 7 : Rat lung lysates
    Lane 8 : Rat heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155992).

  • Flow Cytometry - Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    Flow Cytometry - Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    Flow Cytometry analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling TIP-1 with Purified ab155992 at 1/200 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155992).
  • Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)
    Anti-TIP-1 antibody [EPR7900(2)] - BSA and Azide free (ab213403)

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