Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)
![Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-1-anti-carboxypeptidase-a-antibody-epr2438469-immunohistochemistry-human-pancreas.jpg "Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24384-69] to Carboxypeptidase A
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Carboxypeptidase A antibody [EPR24384-69]
See all Carboxypeptidase A primary antibodies -
Description
Rabbit monoclonal [EPR24384-69] to Carboxypeptidase A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human pancreas tissue lysate; Mouse pancreas tissue lysate; Rat pancreas tissue lysate. IHC-P: Human pancreas tissue; Mouse pancreas tissue; Rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; Rat pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24384-69 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on acinar cells of human pancreas (PMID: 23006325). The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-5-anti-carboxypeptidase-a-antibody-epr2438469-immunohistochemistry-frozen-mouse-pancreas.jpg)
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/100 (5.39 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-447671-anti-carboxypeptidase-a-antibody-epr2438469-western-blot-lysate.jpg)
Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)All lanes : Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044) at 1/1000 dilution
Lane 1 : Human pancreas tissue lysate
Lane 2 : Mouse pancreas tissue lysate
Lane 3 : Rat pancreas tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 33,43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 21098023).
Activation of pro-carboxypeptidase A is initiated by proteolytic cleavage at the C-terminal end of the propeptide by trypsin and chymotrypsin (PMID: 8436102, 21098023).
Exposure time: 3 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-2-anti-carboxypeptidase-a-antibody-epr2438469-immunohistochemistry-mouse-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on acinar cells of mouse pancreas. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-6-anti-carboxypeptidase-a-antibody-epr2438469-immunohistochemistry-frozen-rat-pancreas.jpg)
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/500 (1.078 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution (Green). Positive staining on rat pancreas is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-3-anti-carboxypeptidase-a-antibody-epr2438469-immunohistochemistry-rat-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on acinar cells of rat pancreas. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-4-anti-carboxypeptidase-a-antibody-epr2438469-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)](https://www.abcam.com/ps/products/278/ab278044/Images/ab278044-448069-benefitsofrecombinantantibodies.png)
Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)