Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24384-69] to Carboxypeptidase A
Suitable for: IHC-Fr, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Carboxypeptidase A antibody [EPR24384-69]
See all Carboxypeptidase A primary antibodies
Description

Rabbit monoclonal [EPR24384-69] to Carboxypeptidase A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human pancreas tissue lysate; Mouse pancreas tissue lysate; Rat pancreas tissue lysate. IHC-P: Human pancreas tissue; Mouse pancreas tissue; Rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; Rat pancreas tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24384-69
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Cytoplasmic staining on acinar cells of human pancreas (PMID: 23006325). The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/100 (5.39 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

All lanes : Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044) at 1/1000 dilution

Lane 1 : Human pancreas tissue lysate
Lane 2 : Mouse pancreas tissue lysate
Lane 3 : Rat pancreas tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 33,43 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 21098023).

Activation of pro-carboxypeptidase A is initiated by proteolytic cleavage at the C-terminal end of the propeptide by trypsin and chymotrypsin (PMID: 8436102, 21098023).

Exposure time: 3 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Cytoplasmic staining on acinar cells of mouse pancreas. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/500 (1.078 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) at 1/1000 dilution (Green). Positive staining on rat pancreas is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Cytoplasmic staining on acinar cells of rat pancreas. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044)

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