Human FABP-1 ELISA Kit (ab222875)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.563 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FABP-1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% Inter-assay Sample n Mean SD CV% Overall 9.8% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
1.563 ng/ml - 50 ng/ml -
Recovery
= 97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Rabbit, Cow -
Product overview
Human FABP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222875) is designed for the quantitative measurement of human FABP-1 protein in plasma, serum, saliva, milk, cerebral spinal fluid and cell culture samples.
The assay employs a quantitative sandwich enzyme immunoassay technique that measures human FABP-1 in approximately 4 hours. A polyclonal antibody specific for human FABP-1 has been pre-coated onto a 96-well microplate with removable strips. FABP-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human FABP-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Mitochondrial glutamate oxaloacetate transaminase 2 (FABP-1), also called mitochondrial aspartate aminotransferase (mAspAT) and fatty acid-binding protein, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT is a pyridoxal phosphate-dependent enzyme, which exists in cytoplasmic GOT1 and inner-membrane mitochondrial FABP-1 forms. GOT plays a role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The two enzymes show close homology. FABP-1 is a dimer containing two identical subunits, each 45 kDa with about 401 amino acids. FABP-1 acetylation stimulates the malate–aspartate NADH shuttle activity and oxidative protection. It is also involved in cell proliferation and energy production.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 30X Biotinylated Human FABP-1 1 x 180µl Anti-Human FABP-1 coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human FABP-1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane. - Information by UniProt
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Alternative names
- AATM_HUMAN
- AL022787
- Aspartate aminotransferase
see all -
Database links
- Entrez Gene: 2806 Human
- Omim: 138150 Human
- SwissProt: P00505 Human
- Unigene: 599470 Human
Images
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Human FABP-1 standard curve
Example of human FABP-1 standard curve. Background-subtracted data are graphed.