Mouse PRAS40 Matched Antibody Pair Kit (ab218794)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Mouse PRAS40 Matched Antibody Pair Kit
See all PRAS40 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
24.43 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PRAS40 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PRAS40.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210580. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse PRAS40 Capture Antibody 2 x 50µg 1 x 50µg Mouse PRAS40 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse PRAS40 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. -
Tissue specificity
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293). -
Post-translational
modificationsPhosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1. -
Cellular localization
Cytoplasm > cytosol. Found in the cytosolic fraction of the brain. - Information by UniProt
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Alternative names
- 40 kDa proline rich AKT substrate
- 40 kDa proline-rich AKT substrate
- AKT1 S1
see all -
Database links
- Entrez Gene: 67605 Mouse
- SwissProt: Q9D1F4 Mouse
- Unigene: 148007 Mouse
Images
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Mouse PRAS40 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse PRAS40 Matched Antibody Pair Kit (ab218794)To learn more about the advantages of recombinant antibodies see here.
