Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A11 antibody [EPR11172] (ab180593)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11172] to S100A11
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-S100A11 antibody [EPR11172]
    See all S100A11 primary antibodies

  • Description

    Rabbit monoclonal [EPR11172] to S100A11

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SKBR-3, PC-3 or BxPC-3 lysate, Human ovarian carcinoma tissue, human thyroid carcinoma, BxPC-3 or PC-3 cells. PC-12 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    Anti-S100A11 antibody [EPR11172] (ab180593) at 1/10000 dilution + Sample: SK-BR-3 (human mammary gland adenocarcinoma) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    Diluting and blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] (ab180593)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] (ab180593)

    ab180593 staining S100A11 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/3000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    All lanes : Anti-S100A11 antibody [EPR11172] (ab180593) at 1/5000 dilution

    Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution


    Diluting and blocking buffer: 5% NFDM /TBST

  • Flow Cytometry - Anti-S100A11 antibody [EPR11172] (ab180593)
    Flow Cytometry - Anti-S100A11 antibody [EPR11172] (ab180593)

    Flow cytometry analysis of PC-3 cells using ab180593 at a 1/150 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] (ab180593)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] (ab180593)

    Immunofluorescence analysis of BxPC-3 cells (fixative 4% paraformaldehyde) labeling S100A11 with ab180593 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution. 

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] (ab180593)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] (ab180593)

    ab180593 staining S100A11 in PC-3 (human prostate adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab180593 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Flow Cytometry - Anti-S100A11 antibody [EPR11172] (ab180593)
    Flow Cytometry - Anti-S100A11 antibody [EPR11172] (ab180593)

    ab180593 staining S100A11 in PC-12 (rat adrenal gland pheochromocytoma) cell line by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/180. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] (ab180593)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] (ab180593)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling S100A11 with ab180593 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    Anti-S100A11 antibody [EPR11172] (ab180593) at 1/20000 dilution + BxPC-3 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    Western blot - Anti-S100A11 antibody [EPR11172] (ab180593)
    All lanes : Anti-S100A11 antibody [EPR11172] (ab180593) at 1/5000 dilution

    Lane 1 : SKBR-3 lysate
    Lane 2 : PC-3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • Anti-S100A11 antibody [EPR11172] (ab180593)
    Anti-S100A11 antibody [EPR11172] (ab180593)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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