Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11172] to S100A11 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-S100A11 antibody [EPR11172] - BSA and Azide free
    See all S100A11 primary antibodies

  • Description

    Rabbit monoclonal [EPR11172] to S100A11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human thyroid carcinoma tissue.

  • General notes

    Ab236123 is the carrier-free version of ab180593. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236123 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Flow Cytometry - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

    ab180593 staining S100A11 in PC-12 (rat adrenal gland pheochromocytoma) cell line by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/180. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

    ab180593 staining S100A11 in PC-3 (human prostate adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab180593 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

  • Flow Cytometry - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Flow Cytometry - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

    Flow cytometry analysis of PC-3 cells using ab180593 at a 1/150 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

    Immunofluorescence analysis of BxPC-3 cells (fixative 4% paraformaldehyde) labeling S100A11 with ab180593 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling S100A11 with ab180593 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

    ab180593 staining S100A11 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/3000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180593).

  • Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)
    Anti-S100A11 antibody [EPR11172] - BSA and Azide free (ab236123)

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