Anti-CD62L antibody - C-terminal (ab135792)
Key features and details
- Rabbit polyclonal to CD62L - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD62L antibody - C-terminal
See all CD62L primary antibodies -
Description
Rabbit polyclonal to CD62L - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 343-372 of Human CD62L.
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Positive control
- Jurkat cell line lysate; Human lymph tissue; Jurkat cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab135792 is purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD62L antibody - C-terminal (ab135792)
Flow cytometric analysis of Jurkat cells labelling CD62L with ab135792 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab135792, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated Goat anti-Rabbit antibodies at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62L antibody - C-terminal (ab135792)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph tissue labelling CD62L C-terminal with ab135792 at 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
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Western blot - Anti-CD62L antibody - C-terminal (ab135792)Anti-CD62L antibody - C-terminal (ab135792) at 1/1000 dilution + Jurkat whole cell lysate at 35 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
