Anti-CSF-1-R antibody [EPR20634] (ab239079)
![Anti-CSF-1-R antibody [EPR20634] (ab239079)](https://www.abcam.com/ps/products/239/ab239079/Images/ab239079-318405-anti-mcsf-receptor-antibody-epr20634-immunohistochemistry.jpg "Anti-CSF-1-R antibody [EPR20634] (ab239079)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20634] to CSF-1-R
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CSF-1-R antibody [EPR20634]
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [EPR20634] to CSF-1-R -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human tonsil, lymphoma and placenta lysates. IHC-P: Human colon, tonsil and Hodgkin's lymphoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20634 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20634] (ab239079)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CSF-1-R with ab239079 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining on macrophages in human colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CSF-1-R antibody [EPR20634] (ab239079)](https://www.abcam.com/ps/products/239/ab239079/Images/ab239079-318404-anti-mcsf-receptor-antibody-epr20634-western-blot.jpg)
Western blot - Anti-CSF-1-R antibody [EPR20634] (ab239079)All lanes : Anti-CSF-1-R antibody [EPR20634] (ab239079) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human lymphoma lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20634] (ab239079)](https://www.abcam.com/ps/products/239/ab239079/Images/ab239079-318407-anti-mcsf-receptor-antibody-epr20634-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20634] (ab239079)Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CSF-1-R with ab239079 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining on macrophages in human Hodgkin’s lymphoma is observed (PMID:26066800). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20634] (ab239079)](https://www.abcam.com/ps/products/239/ab239079/Images/ab239079-318406-anti-mcsf-receptor-antibody-epr20634-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20634] (ab239079)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CSF-1-R with ab239079 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining on macrophages in human tonsil is observed (PMID:26066800). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-CSF-1-R antibody [EPR20634] (ab239079)](https://www.abcam.com/ps/products/239/ab239079/Images/ab239079-5-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [EPR20634] (ab239079)
