HRP Anti-IKK alpha antibody [Y463] (ab200415)
![HRP Anti-IKK alpha antibody [Y463] (ab200415)](https://www.abcam.com/ps/products/200/ab200415/Images/ab200415-391732-anti-ikk-alpha-antibody-y463-western-blot-hap1-wt-hap1-chuck-knockout.jpg "HRP Anti-IKK alpha antibody [Y463] (ab200415)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y463] to IKK alpha
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-IKK alpha antibody [Y463]
See all IKK alpha primary antibodies -
Description
HRP Rabbit monoclonal [Y463] to IKK alpha -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi, HeLa and HAP1 cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y463 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-IKK alpha antibody [Y463] (ab200415)All lanes : HRP Anti-IKK alpha antibody [Y463] (ab200415) at 1/5000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CHUK knockout HAP1 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 89 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab200415 was shown to react with IKK alpha (HRP) in wild-type HAP1 cells in western blot. Loss of signal was observed when CHUK knockout sample was used. Membranes were blocked in 2% BSA in TBS-T (0.1% Tween®) before incubation with ab200415 overnight at 4°C at a 1 in 5000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
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![Western blot - HRP Anti-IKK alpha antibody [Y463] (ab200415)](https://www.abcam.com/ps/products/200/ab200415/Images/ab200415-283826-WBab2004151.jpg)
Western blot - HRP Anti-IKK alpha antibody [Y463] (ab200415)All lanes : HRP Anti-IKK alpha antibody [Y463] (ab200415) at 1/5000 dilution
Lane 1 :Daudi whole cell lysate (ab3951)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 3 : Hap1 WT
Lane 4 : IKK alpha knockout HAP1 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200415 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-IKK alpha antibody [Y463] (ab200415)](https://www.abcam.com/ps/products/200/ab200415/Images/ab200415-4-benefits-of-recombinant-antibodies.png)
HRP Anti-IKK alpha antibody [Y463] (ab200415)
