Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278)
Key features and details
- Rabbit polyclonal to PACAP receptor/ADCYAP1R1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PACAP receptor/ADCYAP1R1 antibody
See all PACAP receptor/ADCYAP1R1 primary antibodies -
Description
Rabbit polyclonal to PACAP receptor/ADCYAP1R1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab109076) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human brain; Human liver; Human pancreas; Human small intestine.
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General notes
This product was previously labelled as PACAP receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications SheepCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).Target
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Function
This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. -
Tissue specificity
Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 117 Human
- Omim: 102981 Human
- SwissProt: P41586 Human
- Unigene: 377783 Human
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Alternative names
- ADCYAP1 R1 antibody
- ADCYAP1R 1 antibody
- ADCYAP1R1 antibody
see all
Images
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Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278)All lanes : Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 10 minutesThe band observed at 51 kDa could potentially be a cleaved form of PACAP receptor/ADCYAP1R1 due to the presence of a 20 amino acid signal peptide. A positive signal was also seen in both Human liver and Human pancreas tissue lysates.
Datasheets and documents
References (0)
ab95278 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278)All lanes : Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 10 minutesThe band observed at 51 kDa could potentially be a cleaved form of PACAP receptor/ADCYAP1R1 due to the presence of a 20 amino acid signal peptide. A positive signal was also seen in both Human liver and Human pancreas tissue lysates.
