Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)
![Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-270780-anti-mpg-antibody-epr10959b-western-blot.jpg "Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10959(B)] to MPG/AAG
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MPG/AAG antibody [EPR10959(B)]
See all MPG/AAG primary antibodies -
Description
Rabbit monoclonal [EPR10959(B)] to MPG/AAG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
corresponding to Human MPG/AAG.
Database link: P29372 -
Positive control
- HeLa, 293T and Jurkat cell lysates; Human testis tissue and ovarian carcinoma tissue.
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General notes
This product was previously labelled as MPG
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10959(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MPG/AAG knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab155092 observed at 35 kDa. Red - loading control, ab8245.ab155092 was shown to specifically react with MPG/AAG when MPG/AAG knockout samples were used. Wild-type and MPG/AAG knockout samples were subjected to SDS-PAGE. Ab155092 and ab8245 (loading control to GAPDH) were diluted at 1/10,000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161985-anti-mpg-antibody-epr10959b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161989-anti-mpg-antibody-epr10959b-western-blot.jpg)
Western blot - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)All lanes : Anti-MPG/AAG antibody [EPR10959(B)] (ab155092) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
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![Flow Cytometry - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-276952-ab155092FC1.jpg)
Flow Cytometry - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MPG/AAG with unpurified ab155092 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161988-anti-mpg-antibody-epr10959b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG/AAG with ab155092 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161987-anti-mpg-antibody-epr10959b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG/AAG with ab155092 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161986-anti-mpg-antibody-epr10959b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161984-anti-mpg-antibody-epr10959b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-161983-anti-mpg-antibody-epr10959b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)](https://www.abcam.com/ps/products/155/ab155092/Images/ab155092-12-benefits-of-recombinant-antibodies.png)
Anti-MPG/AAG antibody [EPR10959(B)] (ab155092)
