Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)
![Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)](https://www.abcam.com/ps/products/234/ab234525/Images/ab234525-308568-anti-mcsf-receptor-antibody-epr21885-161-western-blot.jpg "Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21885-161] to CSF-1-R - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free
See all CSF-1-R primary antibodies -
Description
Rabbit monoclonal [EPR21885-161] to CSF-1-R - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung lysate, Mouse spleen and placenta lysate, J774A.1 and RAW 264.7 whole cell lysate.
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General notes
Ab234525 is the carrier-free version of ab221684. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab234525 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21885-161 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)All lanes : Anti-CSF-1-R antibody [EPR21885-161] (ab221684) at 1/1000 dilution
Lane 1 : Rat lung lysate at 20 µg
Lane 2 : Mouse spleen lysate at 20 µg
Lane 3 : Mouse placenta lysate at 20 µg
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 10 µg
Lane 5 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 3 minutes; Lane 5: 32 seconds.
The molecular mass observed is consistent with the literature (PMID 21610095; PMID 20436485; PMID 24362524; PMID: 26066800).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221684).
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![Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)](https://www.abcam.com/ps/products/234/ab234525/Images/ab234525-2-benefits-of-recombinant-antibodies.png)
Anti-CSF-1-R antibody [EPR21885-161] - BSA and Azide free (ab234525)
