Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23529-26] to CSF-1-R
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CSF-1-R antibody [EPR23529-26]
    See all CSF-1-R primary antibodies

  • Description

    Rabbit monoclonal [EPR23529-26] to CSF-1-R

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7, J774A.1 and Mouse spleen, Mouse placenta lysates. IHC-P: Mouse spleen, Mouse liver tissues. ICC/IF: RAW 264.7 cells. IP: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in mouse spleen. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling CSF-1-R with ab254357 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    All lanes : Anti-CSF-1-R antibody [EPR23529-26] (ab254357) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Mouse placenta tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 108 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-2: 70 seconds; Lanes 3-4: 3 minutes.

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:14673177)
    This blot was developed using a higher sensitivity ECL substrate.

     

     

  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

    CSF-1-R was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab254357 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

    Lane 2: ab254357 IP in RAW 264.7 whole cell lysate

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab254357 in RAW 264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 128 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells in mouse liver. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Anti-CSF-1-R antibody [EPR23529-26] (ab254357)
    Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

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