Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23529-26] to CSF-1-R
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse

Product name

Anti-CSF-1-R antibody [EPR23529-26]
See all CSF-1-R primary antibodies
Description

Rabbit monoclonal [EPR23529-26] to CSF-1-R
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: RAW 264.7, J774A.1 and Mouse spleen, Mouse placenta lysates. IHC-P: Mouse spleen, Mouse liver tissues. ICC/IF: RAW 264.7 cells. IP: RAW 264.7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23529-26
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in mouse spleen. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling CSF-1-R with ab254357 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Western blot - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

All lanes : Anti-CSF-1-R antibody [EPR23529-26] (ab254357) at 1/1000 dilution

Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse placenta tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 108 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1-2: 70 seconds; Lanes 3-4: 3 minutes.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:14673177)
This blot was developed using a higher sensitivity ECL substrate.
Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

CSF-1-R was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab254357 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

Lane 2: ab254357 IP in RAW 264.7 whole cell lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab254357 in RAW 264.7 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 128 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells in mouse liver. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Anti-CSF-1-R antibody [EPR23529-26] (ab254357)

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