Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23529-26] to CSF-1-R - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free
    See all CSF-1-R primary antibodies

  • Description

    Rabbit monoclonal [EPR23529-26] to CSF-1-R - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7, J774A.1 and Mouse spleen, Mouse placenta lysates. IHC-P: and Mouse spleen, Mouse liver tissues. ICC/IF: RAW264.7 cell. IP: RAW 264.7 cell.

  • General notes

    ab272049 is the carrier-free version of ab254357. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages in mouse spleen. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
    Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 cells labelling CSF-1-R with ab254357 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

  • Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
    Immunoprecipitation - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

    CSF-1-R was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with ab254357 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254357 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

    Lane 2: ab254357 IP in RAW 264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254357 in RAW 264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 128 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CSF-1-R with ab254357 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells in mouse liver. The section was incubated with ab254357 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254357).

  • Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)
    Anti-CSF-1-R antibody [EPR23529-26] - BSA and Azide free (ab272049)

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