Anti-TRIM47 antibody (ab72234)
Key features and details
- Rabbit polyclonal to TRIM47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM47 antibody -
Description
Rabbit polyclonal to TRIM47 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide from a region between residue 125 and 175 of human TRIM47, NP_258411.2.
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Positive control
- Whole cell lysate from Hela and 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIM47 antibody (ab72234)All lanes : Anti-TRIM47 antibody (ab72234) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 70 kDa
Observed band size: 70,80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-TRIM47 antibody (ab72234)Immunoprecipitation/ Western Blot of TRIM47.
Lane 1: ab72234 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72234 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.