Anti-c-Jun (phospho S73) antibody (ab30620)
Key features and details
- Rabbit polyclonal to c-Jun (phospho S73)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Jun (phospho S73) antibody
See all c-Jun primary antibodies -
Description
Rabbit polyclonal to c-Jun (phospho S73) -
Host species
Rabbit -
Specificity
ab30620 detects endogenous levels of c-Jun only when phosphorylated at Serine 73. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue sections, using 1/50 Phospho c-Jun(Ser73) Antibody (ab30620). Left: untreated sample. Right: sample preincubated with synthesized phosphopeptide.
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All lanes : Anti-c-Jun (phospho S73) antibody (ab30620) at 1/500 dilution
Lane 1 : extracts from untreated Hela cells
Lane 2 : extracts from UV treated Hela cells
Predicted band size: 36 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?