Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6518] to JunB
Suitable for: WB, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-JunB antibody [EPR6518]
See all JunB primary antibodies
Description

Rabbit monoclonal [EPR6518] to JunB
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human JunB (N terminal). The exact sequence is proprietary.
Database link: P17275
Positive control
- WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (K_D)

K_D = 9.30 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6518
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (ab128878)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-JunB antibody [EPR6518] (ab128878)

All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/200 dilution (Purified)

Lane 1 : Rat lung lysates
Lane 2 : Rat spleen lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 43 kDa
why is the actual band size different from the predicted?
Western blot - Anti-JunB antibody [EPR6518] (ab128878)

All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse uterus lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Western blot - Anti-JunB antibody [EPR6518] (ab128878)

Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified) + Human uterus lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-JunB antibody [EPR6518] (ab128878)

ab128878 (purified) at 1:100 dilution (2 µg) immunoprecipitating JunB in HeLa whole cell lysates.

Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+): ab128878 & HeLa whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-JunB antibody [EPR6518] (ab128878)

All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Unpurified)

Lane 1 : MCF-7 lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human uterus cancer lysate
Lane 4 : HeLa lysate
Lane 5 : HACAT lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (ab128878)

Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.
OI-RD Scanning - Anti-JunB antibody [EPR6518] (ab128878)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-JunB antibody [EPR6518] (ab128878)

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