Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JunB antibody [EPR6518] (ab128878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6518] to JunB
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JunB antibody [EPR6518]
    See all JunB primary antibodies

  • Description

    Rabbit monoclonal [EPR6518] to JunB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human JunB (N terminal). The exact sequence is proprietary.
    Database link: P17275

  • Positive control

    • WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (ab128878)
    Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (ab128878)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/200 dilution (Purified)

    Lane 1 : Rat lung lysates
    Lane 2 : Rat spleen lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse uterus lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified) + Human uterus lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-JunB antibody [EPR6518] (ab128878)
    Immunoprecipitation - Anti-JunB antibody [EPR6518] (ab128878)

    ab128878 (purified) at 1:100 dilution (2 µg) immunoprecipitating JunB in HeLa whole cell lysates.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab128878 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    Western blot - Anti-JunB antibody [EPR6518] (ab128878)
    All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Unpurified)

    Lane 1 : MCF-7 lysate
    Lane 2 : Human tonsil lysate
    Lane 3 : Human uterus cancer lysate
    Lane 4 : HeLa lysate
    Lane 5 : HACAT lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (ab128878)
    Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (ab128878)

    Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.

  • OI-RD Scanning - Anti-JunB antibody [EPR6518] (ab128878)
    OI-RD Scanning - Anti-JunB antibody [EPR6518] (ab128878)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-JunB antibody [EPR6518] (ab128878)
    Anti-JunB antibody [EPR6518] (ab128878)

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