Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6518] to JunB - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JunB antibody [EPR6518] - BSA and Azide free
    See all JunB primary antibodies

  • Description

    Rabbit monoclonal [EPR6518] to JunB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells.

  • General notes

    Ab232033 is the carrier-free version of ab128878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232033 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)
    Immunoprecipitation - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)

    ab128878 (purified) at 1:100 dilution (2 µg) immunoprecipitating JunB in HeLa whole cell lysates.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab128878 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878).

  • Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)
    Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878).

  • Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)
    Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)

    Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878).

  • OI-RD Scanning - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)
    OI-RD Scanning - Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878).

  • Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)
    Anti-JunB antibody [EPR6518] - BSA and Azide free (ab232033)

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