Anti-CNBP antibody (ab48027)
Key features and details
- Goat polyclonal to CNBP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CNBP antibody
See all CNBP primary antibodies -
Description
Goat polyclonal to CNBP -
Host species
Goat -
Specificity
No crossreaction expected with CCHC domain containing 13. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey
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Immunogen
Synthetic peptide:
GESGHLARECTIE
, corresponding to C terminal amino acids 162-174 of Human CNBP.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CNBP antibody (ab48027)Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 19 kDa
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Western blot - Anti-CNBP antibody (ab48027) Image courtesy of an anonymous Abreview.All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
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Western blot - Anti-CNBP antibody (ab48027) Image courtesy of an anonymous Abreview.All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC-1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
