Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse PRAS40 Antibody Pair - BSA and Azide free (ab241790)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

Overview

  • Product name

    Mouse PRAS40 Antibody Pair - BSA and Azide free
    See all PRAS40 kits

  • Assay type

    ELISA set

  • Range

    125 pg/ml - 8000 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    The Antibody Pair can be used to quantify Mouse PRAS40. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab210580), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse PRAS40 Capture Antibody (unconjugated) 1 x 100µg
    Mouse PRAS40 Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

  • Tissue specificity

    Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).

  • Post-translational
    modifications

    Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.

  • Cellular localization

    Cytoplasm > cytosol. Found in the cytosolic fraction of the brain.
  • Information by UniProt

  • Alternative names

    • 40 kDa proline rich AKT substrate
    • 40 kDa proline-rich AKT substrate
    • AKT1 S1
    • AKT1 substrate 1
    • AKT1 substrate 1 (proline rich)
    • AKT1S 1
    • AKT1S1
    • AKTS1_HUMAN
    • Lobe
    • MGC2865
    • PRAS
    • PRAS 40
    • PRAS40
    • Proline rich akt substrate
    • Proline rich Akt substrate 40 kDa
    • Proline-rich AKT1 substrate 1
    see all

  • Database links

    Images

    • Sandwich ELISA - Mouse PRAS40 Antibody Pair - BSA and Azide free (ab241790)
      Sandwich ELISA - Mouse PRAS40 Antibody Pair - BSA and Azide free (ab241790)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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