Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCSK9 antibody [EPR7627(2)] (ab181142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7627(2)] to PCSK9
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PCSK9 antibody [EPR7627(2)]
    See all PCSK9 primary antibodies

  • Description

    Rabbit monoclonal [EPR7627(2)] to PCSK9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, BxPC-3 and HepG2 whole cell lysate (ab7900) and Human fetal liver tissue lysate. IP: HepG2 whole cell lysate (ab7900).

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/5000 dilution (purified) + BxPC-3 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62,78 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/20000 dilution (purified)

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62,78 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)

    ab181142 (purified) at 1/50 immunoprecipitating PCSK9 in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg)

    Lane 2 (+): ab181142 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181142 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/10000 dilution (unpurified)

    Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62,78 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Western blot - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/2000 dilution (unpurified) + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 74 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (ab181142)

    Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using unpurified ab181142 at a dilution of 1/50.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • Anti-PCSK9 antibody [EPR7627(2)] (ab181142)
    Anti-PCSK9 antibody [EPR7627(2)] (ab181142)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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