Anti-PCSK9 antibody (ab245361)
Key features and details
- Rabbit polyclonal to PCSK9
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCSK9 antibody
See all PCSK9 primary antibodies -
Description
Rabbit polyclonal to PCSK9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human PCSK9 aa 450-500. The exact sequence is proprietary.
Database link: Q8NBP7 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab245361 has not performed satisfactorily when used for WB of PCSK9 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PCSK9.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245361 was affinity purified using an epitope specific to PCSK9 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-PCSK9 antibody (ab245361)
PCSK9 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245361 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab245361 at 1 µg/ml.
Lane 1: ab245361 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
