Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7627(2)] to PCSK9 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free
    See all PCSK9 primary antibodies

  • Description

    Rabbit monoclonal [EPR7627(2)] to PCSK9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and HepG2 cell lysate.

  • General notes

    Ab238995 is the carrier-free version of ab181142. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)
    Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)

    ab181142 (purified) at 1/50 immunoprecipitating PCSK9 in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg)

    Lane 2 (+): ab181142 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181142 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181142).

  • Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)
    Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)

    Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using unpurified ab181142 at a dilution of 1/50.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181142).

  • Western blot - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)
    Western blot - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)
    All lanes : Anti-PCSK9 antibody [EPR7627(2)] (ab181142) at 1/1000 dilution (unpurified)

    Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 74 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab181142).

  • Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)
    Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (ab238995)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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