Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17827-117] to PCSK9 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free
    See all PCSK9 primary antibodies

  • Description

    Rabbit monoclonal [EPR17827-117] to PCSK9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Hepa1-6 cells. Flow: Hepa1-6 cells. IP: Hepa1-6 whole cell lysate.

  • General notes

    Ab246322 is the carrier-free version of ab185194. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab246322 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Flow Cytometry - Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)
    Flow Cytometry - Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling PCSK9 with ab185194 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185194).

  • Immunoprecipitation - Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)
    Immunoprecipitation - Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)

    PCSK9 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab185194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185194 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: Hepa1-6 whole cell lysate 10 µg (input).
    Lane 2: ab185194 IP in Hepa1-6 whole cell lysate (+).
    Lane 3: Rabitt monoclonal IgG in HePa1-6 whole cell lsyate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

    78-kDa band represents proPCSK9 (PMID: 25210046).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185194).

  • Immunocytochemistry/ Immunofluorescence - Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)
    Immunocytochemistry/ Immunofluorescence - Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PCSK9 with ab185194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185194).

  • Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)
    Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PCSK9 antibody [EPR17827-117] - BSA and Azide free (ab246322)

© 2021 Astana Biomed Group LLP. All rights reserved.