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Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22152-22] to IGFBP3 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free
    See all IGFBP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22152-22] to IGFBP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung primary cell culture supernatant; Mouse serum; Rat serum.

  • General notes

    Ab239789 is the carrier-free version of ab220429. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239789 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)
    Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)

    IGFBP3 was immunoprecipitated from 0.35 mg of mouse serum with ab220429 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220429 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse serum 10 μg (Input).
    Lane 2: ab220429 IP in mouse serum (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20429 in mouse serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Non-glycosylated IGFBP-3 (29 kDa) is detected in mouse serum. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220429).

  • Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)
    Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)

    IGFBP3 was immunoprecipitated from 0.35 mg of rat serum with ab220429 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220429 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Rat serum 10 μg (Input). 
    Lane 2: ab220429 IP in rat serum (+). 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20429 in rat serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Both glycosylated (46 kDa) and non-glycosylated (29 kDa) IGFBP-3 are detected in rat serum. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394; PMID: 9698597).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220429).

  • Western blot - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)
    Western blot - Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)
    All lanes : Anti-IGFBP3 antibody [EPR22152-22] (ab220429) at 1/1000 dilution

    Lane 1 : Mouse lung primary cell culture supernatant at 20 µl
    Lane 2 : Mouse serum at 20 µg
    Lane 3 : Rat serum at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 32 kDa
    Observed band size: 29,46 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 8 seconds, Lane 2: 81 seconds, Lane 3: 3 minutes

    IGFBP3 exists as both glycosylated (46 kDa) and non-glycosylated (29 kDa) form. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394; PMID: 9698597; PMID 22910030).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220429).

     

  • Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)
    Anti-IGFBP3 antibody [EPR22152-22] - BSA and Azide free (ab239789)

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