Anti-IGFBP3 antibody (ab217205)
Key features and details
- Rabbit polyclonal to IGFBP3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-IGFBP3 antibody
See all IGFBP3 primary antibodies -
Description
Rabbit polyclonal to IGFBP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Synthetic peptide within Human IGFBP3 aa 240-296 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P17936 -
Positive control
- Rat brain tissue; Rat kidney and brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (ab217205)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IGFBP3 with ab217205 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP3 antibody (ab217205)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IGFBP3 with ab217205 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated antibody at 1/200 dilution for 40 minutes at 37°C.
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Western blot - Anti-IGFBP3 antibody (ab217205)All lanes : Anti-IGFBP3 antibody (ab217205) at 1/200 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat brain lysate
Secondary
All lanes : Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?12% Gel
