Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
![Anti-IGFBP3 antibody [EPR18680-153] (ab193910)](https://www.abcam.com/ps/products/193/ab193910/Images/ab193910-285403-anti-igfbp3-antibody-epr18680-153-western-blot.jpg "Anti-IGFBP3 antibody [EPR18680-153] (ab193910)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18680-153] to IGFBP3
- Suitable for: WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-IGFBP3 antibody [EPR18680-153]
See all IGFBP3 primary antibodies -
Description
Rabbit monoclonal [EPR18680-153] to IGFBP3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human serum. IP: Human serum.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18680-153 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)Anti-IGFBP3 antibody [EPR18680-153] (ab193910) at 1/1000 dilution + Human serum at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP Detection Reagent (HRP))
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 10 secondsBlocking and dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)](https://www.abcam.com/ps/products/193/ab193910/Images/ab193910-285404-anti-igfbp3-antibody-epr18680-153-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)IGFBP3 was immunoprecipitated from 0.35 mg of human serum with ab193910 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab193910 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human serum 10 μg (Input).
Lane 2: ab193910 IP in human serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193910 in human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The bands at 41 kDa and 44 kDa have previously been documented as IGFBP3 doublets (PMID:16816363).
-
![Anti-IGFBP3 antibody [EPR18680-153] (ab193910)](https://www.abcam.com/ps/products/193/ab193910/Images/ab193910-3-benefits-of-recombinant-antibodies.png)
Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
