Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 17, 2021

Anti-IGFBP3 antibody [EPR22152-22] (ab220429)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22152-22] to IGFBP3
Suitable for: WB, IP
Reacts with: Mouse, Rat

Product name

Anti-IGFBP3 antibody [EPR22152-22]
See all IGFBP3 primary antibodies
Description

Rabbit monoclonal [EPR22152-22] to IGFBP3
Host species

Rabbit
Tested applications

Suitable for: WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse IGFBP3 recombinant protein (aa22-291); Mouse lung primary cell culture supernatant; Mouse serum; Rat serum. IP: Rat serum; Mouse serum.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22152-22
Isotype

IgG
Research areas

Western blot - Anti-IGFBP3 antibody [EPR22152-22] (ab220429)

All lanes : Anti-IGFBP3 antibody [EPR22152-22] (ab220429) at 1/1000 dilution

Lane 1 : Mouse lung primary cell culture supernatant at 20 µl
Lane 2 : Mouse serum at 20 µg
Lane 3 : Rat serum at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 32 kDa
Observed band size: 29,46 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 8 seconds, Lane 2: 81 seconds, Lane 3: 3 minutes

IGFBP3 exists as both glycosylated (46 kDa) and non-glycosylated (29 kDa) form. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394; PMID: 9698597; PMID 22910030).
Western blot - Anti-IGFBP3 antibody [EPR22152-22] (ab220429)

Anti-IGFBP3 antibody [EPR22152-22] (ab220429) at 1/1000 dilution + Mouse IGFBP3 recombinant protein (aa22-291) at 0.02 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 32 kDa
Observed band size: 29,46 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The recombinant fragment was expressed in a mammalian expression system.
Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] (ab220429)

IGFBP3 was immunoprecipitated from 0.35 mg of mouse serum with ab220429 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220429 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Mouse serum 10 μg (Input).
Lane 2: ab220429 IP in mouse serum (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20429 in mouse serum.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.

Non-glycosylated IGFBP-3 (29 kDa) is detected in mouse serum. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394).
Immunoprecipitation - Anti-IGFBP3 antibody [EPR22152-22] (ab220429)

IGFBP3 was immunoprecipitated from 0.35 mg of rat serum with ab220429 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220429 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Rat serum 10 μg (Input).
Lane 2: ab220429 IP in rat serum (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20429 in rat serum.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.

Both glycosylated (46 kDa) and non-glycosylated (29 kDa) IGFBP-3 are detected in rat serum. The molecular weight observed is consistent with what has been described in the literature (PMID 10352394; PMID: 9698597).
Anti-IGFBP3 antibody [EPR22152-22] (ab220429)

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