Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)
![Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)](https://www.abcam.com/ps/products/224/ab224530/Images/ab224530-2-ab1939102854041939102.jpg "Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18680-153] to IGFBP3 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free
See all IGFBP3 primary antibodies -
Description
Rabbit monoclonal [EPR18680-153] to IGFBP3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human serum.
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General notes
Ab224530 is the carrier-free version of ab193910. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab224530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18680-153 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)
IGFBP3 was immunoprecipitated from 0.35 mg of human serum with ab193910 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab193910 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human serum 10 μg (Input).
Lane 2: ab193910 IP in human serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193910 in human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The bands at 41 kDa and 44 kDa have previously been documented as IGFBP3 doublets (PMID:16816363).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193910).
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![Western blot - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)](https://www.abcam.com/ps/products/224/ab224530/Images/ab224530-285471-193910.jpg)
Western blot - Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)Anti-IGFBP3 antibody [EPR18680-153] (ab193910) at 1/1000 dilution + Human serum at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP secondary antibody (HRP))
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 10 secondsBlocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193910).
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![Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)](https://www.abcam.com/ps/products/224/ab224530/Images/ab224530-3-benefits-of-recombinant-antibodies.png)
Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)
