Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19878] to CEACAM1 + CEACAM6
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-CEACAM1 + CEACAM6 antibody [EPR19878]
See all CEACAM1+CEACAM6 primary antibodies
Description

Rabbit monoclonal [EPR19878] to CEACAM1 + CEACAM6
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment within Human CEACAM1 aa 1-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P13688

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Positive control
- WB: Human CEACAM1 recombinant protein; human CEACAM6 recombinant protein; BxPC-3, HepG2, A549 and HT-29 whole cell lysates; human kidney and fetal liver lysates. IHC-P: Human colon and liver cancer tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19878
Isotype

IgG
Research areas

Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

Lane 1 : Human CEACAM1 recombinant protein
Lane 2 : Human CEACAM5 recombinant protein
Lane 3 : Human CEACAM6 recombinant protein
Lane 4 : Human CEACAM7 recombinant protein
Lane 5 : Human CEACAM8 recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 57 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

Human CEACAM1 recombinant protein contains aa35-428 with GST-tag and His-Tag®. Human CEACAM5, CEACAM6, CEACAM7, CEACAM8 recombinant proteins contain aa35-422, aa35-326, aa35-326 and aa35-335 respectively, and all with a His-Tag®.
Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 2 : Human kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 57 kDa
Observed band size: 110-150,75-90 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, PMID: 10751340, PMID: 17081782).
Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 57 kDa
Observed band size: 110-150 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 minute; Lane 2: 3 seconds; Lane 3: 30 seconds; Lane 4: 15 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, PMID: 10751340, PMID: 17081782).
Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

All lanes : Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 57 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

HUVEC does not express CEACAM 6 and PC-3 does not express CEACAM 1 (PMID: 22918079, PMID: 10359532).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Brush border staining on human colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on human liver cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

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