Anti-DB1 antibody [ZNF5J141] (ab50970)
![Anti-DB1 antibody [ZNF5J141] (ab50970)](https://www.abcam.com/ps/products/50/ab50970/Images/ab50970-62772-anti-db1-antibody-znf5j141-western-blot.gif "Anti-DB1 antibody [ZNF5J141] (ab50970)")
Key features and details
- Mouse monoclonal [ZNF5J141] to DB1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-DB1 antibody [ZNF5J141]
See all DB1 primary antibodies -
Description
Mouse monoclonal [ZNF5J141] to DB1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human DB1.
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Positive control
- HeLa cells and whole cell lysate and NIH3T3 whole cell lysate
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General notes
Previously labelled as ZNF161.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50970 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
ZNF5J141 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry - Anti-DB1 antibody [ZNF5J141] (ab50970)](https://www.abcam.com/ps/products/50/ab50970/Images/ab50970-62771-anti-db1-antibody-znf5j141-immunocytochemistry.gif)
Immunocytochemistry - Anti-DB1 antibody [ZNF5J141] (ab50970)ab50970 at 1/1 dilution staining human DB1 in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat Anti-mouse IgG at 1/200 as secondary.
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![Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)](https://www.abcam.com/ps/products/50/ab50970/Images/ab50970-62770-anti-db1-antibody-znf5j141-western-blot.gif)
Western blot - Anti-DB1 antibody [ZNF5J141] (ab50970)Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
