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Anti-DB1 antibody (ab68278)

Price and availability

274 732 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-DB1 antibody (ab68278)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DB1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DB1 antibody
    See all DB1 primary antibodies
  • Description

    Rabbit polyclonal to DB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human DB1 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab129953)

  • Positive control

    • This antibody gave a positive signal in both Human Skeletal Muscle and Human Kidney tissue lysates.
  • General notes

    Previously labelled as ZNF161. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Rabbit
Chicken
Chimpanzee
Gorilla
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
  • Tissue specificity

    Ubiquitously expressed. Highest levels in skeletal muscle and kidney.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q14119 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7716 Human
    • Entrez Gene: 22344 Mouse
    • Entrez Gene: 287615 Rat
    • Omim: 606747 Human
    • SwissProt: Q14119 Human
    • Unigene: 463569 Human
    • Alternative names

      • DB1 antibody
      • MGC82370 antibody
      • Putative transcription factor DB1 antibody
      • Vascular endothelial zinc finger 1 antibody
      • VEZF1 antibody
      • VEZF1_HUMAN antibody
      • Zinc finger protein 161 antibody
      • ZNF161 antibody
      see all

    Images

    • Western blot - Anti-DB1 antibody (ab68278)
      Western blot - Anti-DB1 antibody (ab68278)
      All lanes : Anti-DB1 antibody (ab68278) at 1 µg/ml

      Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
      Lane 2 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 56 kDa
      Additional bands at: 29 kDa, 48 kDa, 87 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab68278? Please let us know so that we can cite the reference in this datasheet.

    ab68278 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-DB1 antibody (ab68278)
      Western blot - Anti-DB1 antibody (ab68278)
      All lanes : Anti-DB1 antibody (ab68278) at 1 µg/ml

      Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
      Lane 2 : Human kidney tissue lysate - total protein (ab30203)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 56 kDa
      Additional bands at: 29 kDa, 48 kDa, 87 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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