Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13233-60] to ARHGAP25
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ARHGAP25 antibody [EPR13233-60]
    See all ARHGAP25 primary antibodies

  • Description

    Rabbit monoclonal [EPR13233-60] to ARHGAP25

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, Raji and Jurkat cell lysates; Human tonsil tissue lysate; Human stomach and hepatocellular carcinoma tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13233-60

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    Western blot - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    All lanes : Anti-ARHGAP25 antibody [EPR13233-60] (ab192020) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 77 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    Western blot - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    All lanes : Anti-ARHGAP25 antibody [EPR13233-60] (ab192020) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ARHGAP25 using ab192020 at 1/200 dilution (5 μg/mL). A prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab192020.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling ARHGAP25 using ab192020 at 1/200 dilution (5 μg/mL). A prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab192020.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)
    Anti-ARHGAP25 antibody [EPR13233-60] (ab192020)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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