Human PIP3E Matched Antibody Pair Kit (ab218799)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Human PIP3E Matched Antibody Pair Kit
See all IPCEF1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
27 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human PIP3E Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human PIP3E.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214573. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human PIP3E Capture Antibody 2 x 50µg 1 x 50µg Human PIP3E Detector Antibody 2 x 12.5µg 1 x 12.5µg Human PIP3E Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner. -
Sequence similarities
Contains 1 PH domain. -
Cellular localization
Cytoplasm. Cell membrane. Translocated with PSCD2 to the plasma membrane upon epidermal growth factor (EGF) stimulation. - Information by UniProt
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Alternative names
- ICEF1_HUMAN
- Interaction protein for cytohesin exchange factors 1
- Interactor protein for cytohesin exchange factors 1
see all -
Database links
- Entrez Gene: 26034 Human
- SwissProt: Q8WWN9 Human
- Unigene: 146100 Human
Images
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Human PIP3E standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human PIP3E Matched Antibody Pair Kit (ab218799)To learn more about the advantages of recombinant antibodies see here.
