Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Armenian hamster monoclonal [JH-1] to p115-RhoGEF - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free
    See all p115-RhoGEF primary antibodies

  • Description

    Armenian hamster monoclonal [JH-1] to p115-RhoGEF - BSA and Azide free

  • Host species

    Armenian hamster

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse p115-RhoGEF. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q61210

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: A20, EL4, PC-12 and C6 whole cell lysates. ICC/IF: A20 and EL4 cells. Flow Cyt: EL4 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255755 is the carrier-free version of ab243248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)
    Western blot - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)
    All lanes : Anti-p115-RhoGEF antibody [JH-1] (ab243248) at 1/1000 dilution

    Lane 1 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
    Lane 2 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1/5000 dilution

    Predicted band size: 102 kDa
    Observed band size: 115 kDa
    why is the actual band size different from the predicted?



    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11384980).

    Lysates should be made freshly and used in WB immediately.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1/2:3 seconds; Lane 3:15 seconds; Lane 4:37 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

  • Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)
    Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/50 dilution, followed by ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A20 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab243248 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab173003 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

  • Flow Cytometry - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)
    Flow Cytometry - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL-4 (mouse lymphoma T lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/40 dilution (1µg) (Red) compared with an Armenian hamster monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab243248.

  • Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)
    Immunocytochemistry/ Immunofluorescence - Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/50 dilution, followed by ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in EL4 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab243248 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab173003 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

  • Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)
    Anti-p115-RhoGEF antibody [JH-1] - BSA and Azide free (ab255755)

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