Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Key features and details

Mouse monoclonal [OTI3H7] to Acid sphingomyelinase - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free
See all Acid sphingomyelinase primary antibodies
Description

Mouse monoclonal [OTI3H7] to Acid sphingomyelinase - BSA and Azide free
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human Acid sphingomyelinase aa 50-350. NP_000534 Produced in E.coli.
Database link: P17405

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Positive control
- IHC-P: Human breast adenocarcinoma, lymphoma, colon adenocarcinoma, lung carcinoma, endometrium adenocarcinoma, liver carcinoma, thyroid carcinoma, prostate carcinoma, ovary adenocarcinoma, kidney carcinoma, pancreas, ovary, lymph node, lung, colon, endometrium, liver, prostate, bladder, kidney and tonsil tissue. WB: pCMV6-ENTRY Acid sphingomyelinase cDNA transfected HEK-293T cell lysate.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.3
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

OTI3H7
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human tonsil tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Western blot - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

All lanes : Same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T cell lysate
Lane 2 : pCMV6-ENTRY Acid sphingomyelinase cDNA transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 70 kDa

Cells were transfected with cDNA for 48 hrs and lysed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human thyroid carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human prostate tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human prostate carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human pancreas tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human ovary tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human ovary adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lymphoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lymph node tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lung tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human lung carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human liver tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human liver carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human kidney tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human kidney carcinoma tissue stained for Acid sphingomyelinase aab273656 at 1/150 diution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human endometrium tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human colon tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human colon adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human breast adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (ab273656)

Paraffin-embedded human bladder tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

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