Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y289] to p63 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-p63 antibody [Y289] - BSA and Azide free
    See all p63 primary antibodies

  • Description

    Rabbit monoclonal [Y289] to p63 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody should detect both isoforms 2,9,10,11 and 12 of p63. 

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab247245 reacts with an epitope located in the C terminal region of p63.

  • General notes

    ab247245 is the carrier-free version of ab32353 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247245 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Western blot - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Anti-p63 antibody [Y289] (ab32353) at 1/4000 dilution (unpurified) + A431 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 77 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32353, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Flow Cytometry - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)

    This data was developed using ab32353, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling p63 with purified ab32353
    at 1/500 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Western blot - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Western blot - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Anti-p63 antibody [Y289] (ab32353) at 1/10000 dilution (purified) + A431 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 77 kDa
    Observed band size: 73 kDa why is the actual band size different from the predicted?



    This data was developed using ab32353, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    This data was developed using ab32353, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular skin tissue labelling p63 with purified ab32353 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
  • Immunocytochemistry - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Immunocytochemistry - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    This data was developed using ab32353, the same antibody clone in a different buffer formulation.Immunocytochemsitry/Immunofluorescence analysis of A431 cells labelling p63 (red) with purified ab32353 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
  • Immunocytochemistry - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Immunocytochemistry - Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    This data was developed using ab32353, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling p63 with unpurified ab32353 at 1/250 dilution.
  • Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)
    Anti-p63 antibody [Y289] - BSA and Azide free (ab247245)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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