Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845) -
Host species
Rabbit -
Specificity
ab76321 only detects Glutamate Receptor 1 (AMPA subtype) phosphorylated on Serine 845. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) (phospho S845).
-
Positive control
- WB: Human fetal, rat and mouse brain tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2148 -
Isotype
IgG -
Research areas
Images
-
Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) labelling Glutamate Receptor 1 (AMPA subtype) (phospho S845) with ab76321 at 1/1000 in 5% NFDM/TBST. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Exposed for 3 minutes.
Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS845) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide
-
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution
Lane 1 : Mouse hippocampus whole cell lysates
Lane 2 : Mouse hippocampus whole cell lysates. The membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocked in 5% NFDM/TBST
-
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution
Lane 1 : Human cerebellum whole cell lysates
Lane 2 : Human cerebellum whole cell lysates. The membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocked with 5% NFDM/TBST
-
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1000 cells (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST.
Diluting buffer: 1%BSA /TBST.
-
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)
Lane 1 : Mouse brain tissue lysate, untreated
Lane 2 : Mouse brain tissue lysate, treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST.
Diluting buffer: 1%BSA /TBST.
-
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)
Lane 1 : Rat brain tissue lysate, untreated
Lane 2 : Rat brain tissue lysate, treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST.
Diluting buffer: 1%BSA /TBST.
-
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (unpurified)
Lane 1 : Rat brain tissue lysate, untreated
Lane 2 : Rat brain tissue lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
-