Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype)
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479]
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies

  • Description

    Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab219618)

  • Positive control

    • WB: Human, Mouse and Rat brain lysates. IP: Mouse brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution

    Lane 1 : Human brain lysates prepared in RIPA lysis method
    Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
    Lane 3 : Rat brain lysates prepared in RIPA lysis method
    Lane 4 : Rat brain lysates prepared in 1%SDS Hot lysis method
    Lane 5 : Mouse brain lysates prepared in RIPA lysis method
    Lane 6 : Mouse brain lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Exposure times:

    Lanes 1 and 2: 180 seconds
    Lanes 3 and 4: 50 seconds
    Lanes 5 and 6: 60 seconds

    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution (unpurified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 102 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

    ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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