Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Key features and details
- Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal -
Host species
Mouse -
Specificity
Does not cross- react with GluR2. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Fusion protein corresponding to Rat Glutamate Receptor 1 (AMPA subtype) aa 1-389 (N terminal). within extracellular domain
Sequence:MPYIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ EALISIIDHY KWQTFVYIYD ADRGLSVLQR VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV VVDCESERLN AILGQIVKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV TGFQLVNYTD TIPARIMQQW RTSDSRDHTR VDWKRPKYTS ALTYDGVKVM AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFV
Database link: P19490 -
Positive control
- WB: Rat brain membrane lysate
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General notes
The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N355/1 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.
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Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/2000 dilution + Rat Brain membrane lysate at 10 µg
Secondary
Goat Anti-Mouse HRP at 1/200 dilution
Predicted band size: 102 kDa