D 2 Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 10 µM
Overview
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Product name
D 2 Hydroxyglutarate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
10 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG) in urine, cell and tissue lysates. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 µM in various samples. This product is specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate.
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Notes
In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of αketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests D2HG Assay Buffer 1 x 20ml D2HG Enzyme 1 vial D2HG Substrate Mix 1 vial D2HG Standard 1 vial
Images
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D-2-Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)
Measurement of D-2-Hydroxyglutarate in different cell lysates: 3T3 (80 µg; mouse), Jurkat (12 µg), HeLa (15 µg), MDA-MB-468 (6 µg), and HepG2 (2 μg).
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D-2-Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)Measurement of D-2-Hydroxyglutarate in rat liver lysate (120 µg) and rat kidney lysate (240 µg).